NR 809.334(2)(a)(a) The time from sample collection to initiation of analysis may not exceed 30 hours unless the condition of par. (b) are met. In all cases, samples should be analyzed as soon after collection as possible. NR 809.334(2)(b)(b) The department may approve on a case-by-case basis the holding of an E. coli sample for up to 48 hours between sample collection and initiation of analysis if the department determines that analyzing an E. coli sample within 30 hours is not feasible. E. coli samples held between 30 to 48 hours shall be analyzed by the Colilert reagent version of Standard Method 9223B as listed in sub. (2), Table I. NR 809.334(2)(c)(c) Water suppliers shall maintain samples between 0 °C and 10 °C during storage and transit to the laboratory. 1Standard Methods for the Examination of Water and Wastewater, 20th edition (1998). Available from American Public Health Association, 800 I Street, NW., Washington, DC 20001-3710.
2Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street, NW., Washington, DC 20001-3710.
3Standard Methods for the Examination of Water and Wastewater, 22nd edition (2012). Available from American Public Health Association, 800 I Street NW., Washington, DC 20001-3710.
4Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used. 5 Multiple-tube and multi-well enumerative formats for this method are approved for use in presence-absence determination under this regulation.
6 ColisureÒ results may be read after an incubation time of 24 hours.
7EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be used for E. coli testing step as described in §141.21(f)(6)(i) or (ii) after use of Standard Methods 9221 B, 9221 D, 9222 B, or 9222 C.
8EPA Method 1600: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-â-D-Glucoside Agar (mEI) EPA 821-R-02-022 (September 2002) is an approved variation of Standard Method 9230C. The method is available at http://www.epa.gov/nerlcwww/1600sp02.pdf or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460. The holding time and temperature for ground water samples are specified in footnote 1 above, rather than as specified in Section 8 of EPA Method 1600. 9Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092. Preparation and use of the medium is set forth in the article “Evaluation of Enterolert for Enumeration of Enterococci in Recreational Waters,” by Budnick, G.E., Howard, R.T., and Mayo, D.R., 1996, Applied and Environmental Microbiology, 62:3881-3884.
10 Charm Sciences Inc. “Fast Phage Test Procedure. Presence/Absence for Coliphage in Ground Water with Same Day Positive Prediction”. Version 009. November 2012. 659 Andover Street, Lawrence, MA 01843. Available at www.charmsciences.com. 11EPA Method 1601: Male-specific (F+) and Somatic Coliphage in Water by Two-step Enrichment Procedure; April 2001, EPA 821-R-01-030. Method is available at http://www.epa.gov/nerlcwww/1601ap01.pdf or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460. 12EPA Method 1602: Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure; April 2001, EPA 821-R-01-029. Method is available at http://www.epa.gov/nerlcwww/1602ap01.pdf or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460. NR 809.334 HistoryHistory: CR 09-073: cr. Register November 2010 No. 659, eff. 12-1-10; CR 15-049: am. (1), (2) (intro.), r. and recr. (2) Table I Register March 2016 No. 723, eff. 4-1-16. NR 809.335NR 809.335 Approved laboratories for surface water source monitoring. NR 809.335(1)(1) Cryptosporidium. Water suppliers shall have Cryptosporidium samples analyzed by a laboratory that is approved under EPA’s Laboratory Quality Assurance Evaluation Program for Analysis of Cryptosporidium in Water or a laboratory that has been certified for Cryptosporidium analysis by an equivalent department laboratory certification program. NR 809.335(2)(2) E. coli. Any laboratory certified by the EPA, The NELAC Institute (TNI), or the department of agriculture, trade and consumer protection for total coliform or E. coli. analysis under s. NR 809.323 (1) (c), Table F is approved for E. coli analysis under this section when the laboratory uses the same technique for E. coli that the laboratory uses for s. NR 809.334 (2), Table I. NR 809.335(3)(3) Turbidity. Measurements of turbidity shall be made by a party approved by the department. NR 809.336NR 809.336 Reporting source water monitoring results. NR 809.336(1)(1) Water suppliers shall report results from the source water monitoring required under s. NR 809.331 (1) and (2) no later than 10 days after the end of the first month following the month when the sample is collected. NR 809.336(1)(a)(a) All water suppliers for public water systems serving at least 10,000 people shall report the results from the initial source water monitoring required under s. NR 809.331 (1) to EPA. NR 809.336(1)(b)(b) If a water supplier is unable to report monitoring results electronically, the water supplier may use an alternative approach for reporting monitoring results that EPA approves. NR 809.336(2)(2) Water suppliers for public water systems serving fewer than 10,000 people shall report results from the initial source water monitoring required under s. NR 809.331 (1) to the department. NR 809.336(3)(3) All water suppliers shall report results from the second round of source water monitoring required under s. NR 809.331 (2) to the department.