NR 809.323 NoteNote: Copies of the documents listed in the footnotes may be obtained from the sources listed in the footnotes. Copies may be inspected at EPA’s Drinking Water Docket, EPA West, 1301 Constitution Avenue, NW., Room B102, Washington DC 20460, Telephone: 202-566-2426, or at the National Archives and Records Administration (NARA). For information on the availability of this material at NARA, call 202-741-6030, or go to: http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html.

1 Methods are described in Standard Methods for the Examination of Water and Wastewater 20th edition (1998) and copies may be obtained from the American Public Health Association, 1015 Fifteenth Street, NW., Washington, DC 20005-2605.

3 EPA Method 1604: Total Coliforms and Escherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (MI Medium); September 2002, EPA 821-R-02-024. Method is available at http://www.epa.gov/microbes/documents/1604sp02.pdf or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

4 A description of the m-ColiBlue24 Test, “Total Coliforms and E. coli Membrane Filtration Method with m-ColiBlue24Ò Broth,” Method No. 10029 Revision 2, August 17, 1999, is available from Hach Company,100 Dayton Ave., Ames, IA 50010 or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

5 A description of the E*Colite Test, “Charm E*Colite Presence/Absence Test for Detection and Identification of Coliform Bacteria and Escherichia coli in Drinking Water,” January 9, 1998, is available from Charm Sciences, Inc., 659 Andover St., Lawrence, MA 01843-1032 or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

6 EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be used for E. coli testing step as described in Sec. 141.21(f)(6)(i) or (ii) after use of Standard Methods 9221 B, 9221 D, 9222 B, or 9222 C.

7 EPA Method 1600: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-[beta]-D-Glucoside Agar (mEI) EPA 821-R- 02-022 (September 2002) is an approved variation of Standard Method 9230C. The method is available at http://www.epa.gov/microbes/documents/1600sp02.pdf or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

8 Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092. Preparation and use of the medium is set forth in the article “Evaluation of Enterolert for Enumeration of Enterococci in Recreational Waters,” by Budnick, G.E., Howard, R.T., and Mayo, D.R., 1996, Applied and Environmental Microbiology, 62:3881-3884.

9 EPA Method 1601: Male-specific (F+) and Somatic Coliphage in Water by Two-step Enrichment Procedure; April 2001, EPA 821-R-01-030. Method is available at http://www.epa.gov/microbes/documents/1601ap01.pdf or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

10 EPA Method 1602: Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure; April 2001, EPA 821-R-01-029. Method is available at http://www.epa.gov/microbes/documents/1602ap01.pdf or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

NR 809.323(2)(a)(a) The department may invalidate an E. coli-positive groundwater source sample collected under s. NR 809.325 (2) under the conditions specified in subd. 1. or 2.

NR 809.323(2)(a)1.1. The water supplier provides the department with written notice from the laboratory that improper sample analysis occurred.

NR 809.323(2)(a)2.2. The department determines and documents in writing that there is substantial evidence that an E. coli-positive groundwater source sample is not related to source water quality.

NR 809.323(2)(b)(b) If the department invalidates an E. coli-positive groundwater source sample, the water supplier shall collect another source water sample under s. NR 809.325 (2) no later than 24 hours after being notified by the department that the sample has been invalidated. The water supplier shall have the sample analyzed for E. coli using the analytical methods in s. NR 809.323 (1) (b) Table G.

NR 809.323(2)(c)(c) The department may extend the 24-hour time limit on a case-by-case basis if the water supplier cannot collect the source water sample within the 24-hour time limit due to circumstances beyond the water supplier’s control. In the case of an extension, the department shall specify in writing how much time the water supplier has to collect the sample.

NR 809.323 HistoryHistory: CR 09-073: cr. Register November 2010 No. 659, eff. 12-1-10; CR 15-049: am. (1) (a), (b), (d) Table G, (2) (title), (a), (b) Register March 2016 No. 723, eff. 4-1-16.

NR 809.325(1)(a)(a) Any groundwater source sample required under sub. (2) shall be collected at a location prior to any treatment of the groundwater source unless the department approves a sampling location after treatment.

NR 809.325(1)(b)(b) If the public water system’s configuration does not allow for sampling at the well itself, the water supplier may collect a sample at a department-approved location to meet the requirements of par. (a), if the sample is representative of the source water quality of that well.

NR 809.325(1)(c)(c) Source water samples taken in response to positive total coliform samples collected under s. NR 809.31 shall be referred to as triggered source water samples.

NR 809.325(2)(a)(a) Water suppliers for groundwater systems shall conduct triggered source water monitoring if all of the following conditions exist:

NR 809.325(2)(a)1.1. The public water system does not provide at least 4-log treatment of viruses before or at the first customer for each groundwater source.

NR 809.325(2)(a)2.2. The public water system is notified that a sample collected under s. NR 809.31 (1) is total coliform-positive and the sample is not invalidated under s. NR 809.31 (3).