NR 809.31 Note Note: Generally, enumeration methods such as membrane filter, numeric enzyme substrate, or 5 tube fermentation tests and heterotrophic plate counts of the raw, settled and finished water on an established schedule will be necessary to meet this requirement.

NR 809.31(7) (7) Heterotrophic plate counts. At all waterworks which have a potential for high total bacteria levels because of the water quality, the method of treatment, chemical addition or other cause, the department may require heterotrophic plate counts pursuant to an established schedule. Analyses shall be conducted in accordance with the analytical requirements in s. NR 809.311 (1), Table F.

NR 809.31(9) (9) Monitoring Violation.

NR 809.31(9)(a) (a) Failure to take every required routine or additional routine sample in a compliance period is a monitoring violation.

NR 809.31(9)(b) (b) Failure to analyze for E. coli following a total coliform-positive routine sample is a monitoring violation.

NR 809.31(10) (10) Reporting Violation.

NR 809.31(10)(a) (a) Failure to submit a monitoring report or completed assessment form after a water supplier properly conducts monitoring or assessment in a timely manner is a reporting violation.

NR 809.31(10)(b) (b) Failure to notify the department following an E. coli-positive sample as required by sub. (4) in a timely manner is a reporting violation.

NR 809.31(10)(c) (c) Failure to submit certification of completion of department-approved start-up procedure by a seasonal system is a reporting violation.

NR 809.31 History History: CR 09-073: cr. Register November 2010 No. 659, eff. 12-1-10; CR 15-049: am. (1) (title), (a), cr. (1) (ag), (ar), renum. (1) (b) 1. to (b) and am., r. (1) (b) 2., renum. (1) (c) to (d) 4. and am., renum. (1) (d) to (c) and am., cr. (1) (de), (dg), (dr), am. (1) (e), cr. (1) (eg), (er), am. (1) (f), (g), (2) (a), renum. (2) (b) to (b) (intro.) and am., cr. (2) (b) 1. to 3., am. (2) (c) to (e), r. and recr. (2) (f) (intro.), am. (2) (f) 1., 2., cr. (2) (f) 3., am. (2) (h), cr. (2m), am. (3) (b) 2., 3., (c), (4) to (6), cr. (9), (10) Register March 2016 No. 723, eff. 4-1-16; corrections in (1) (d) 2. (intro.), (2) (a) (intro.), (b) (intro.), (f) (intro.), (2m) (d) (intro.), (e), (3) (b) 2. made under s. 35.17, Stats., Register March 2016 No. 723; correction in (1) (ag), (ar) made under s. 13.92 (4) (b) 7., Stats., Register July 2022 No. 799.

NR 809.311 NR 809.311 Analytical requirements for microbiological contaminants.

NR 809.311(1) (1) Analytical methods. Analyses conducted to determine compliance with s. NR 809.31 shall be made in accordance with methods listed in Table F.

NR 809.311(2) (2) Sample volume. The standard sample volume required for total coliform analysis, regardless of analytical method used, is 100 ml.

NR 809.311(3) (3) Sample collection preservation and holding time.

NR 809.311(3)(a) (a) Sample collection for microbiological contaminants under s. NR 809.30 shall be conducted using the sample preservation, containers and maximum holding time procedures specified in par. (b).

NR 809.311(3)(b) (b) Sample preservation requirements and holding times for microbiological samples are specified in subds. 1. and 2. In all cases, samples shall be analyzed as soon after collection as possible.

NR 809.311(3)(b)1. 1. Total Coliform and E. coli samples are not required to be preserved by cooling to 10° C, however it is encouraged during transit. Sodium thiosulfate shall be added to the sample container prior to adding water containing chlorine and the holding time shall be 30 hours.

NR 809.311(3)(b)2. 2. Heterotrophs may be either held at room temperature with a holding time of 6 hours or preserved by cooling to 4° C with a holding time of 24 hours.

NR 809.311(4) (4) Laboratory certification. Analyses under this section shall only be conducted by laboratories that have received certification under ch. ATCP 77 or approval by EPA.

NR 809.311(5) (5) Presence or absence. Water suppliers need only determine the presence or absence of total coliforms; a determination of total coliform density is not required.

NR 809.311 Note Note: The coliform density may, however, be helpful in selecting a remedial option.

NR 809.311(6) (6) Required methods. Samples collected to determine compliance with s. NR 809.30 (1) shall be analyzed by enzyme substrate test methods.

NR 809.311(7) (7) Other methods. The department may approve, on a case-by-case basis, other methods for use in determining compliance with s. NR 809.30 (1) if they have been approved by EPA.

NR 809.311(8) (8) Sample integrity. If chlorine odor is present in a drinking water sample the laboratory shall test the sample for the presence of chlorine. If chlorine is detected in the sample, the laboratory shall reject the sample for analysis, based on the improper preservation. The water supplier shall submit a new sample for analysis to replace the rejected sample.

- See PDF for table

1 Standard Methods for the Examination of Water and Wastewater, 20th edition (1998). Available from American Public Health Association, 800 I Street, NW., Washington, DC 20001-3710.

2 Standard Methods for the Examination of Water and Wastewater, 21st edition (2005). Available from American Public Health Association, 800 I Street, NW., Washington, DC 20001-3710.

3 Standard Methods for the Examination of Water and Wastewater, 22nd edition (2012). Available from American Public Health Association, 800 I Street NW., Washington, DC 20001-3710.

4 Standard Methods Online are available at http://www.standardmethods.org. The year in which each method was approved by the Standard Methods Committee is designated by the last two digits in the method number. The methods listed are the only online versions that may be used.