NR 809.32(3) (3) Requirements. All public water systems using groundwater as a source shall comply with the following requirements:

NR 809.32(3)(a) (a) Provide all information required by the department for sanitary surveys conducted under s. NR 809.35.

NR 809.32(3)(b) (b) Groundwater systems that do not treat all of their groundwater to at least 4-log treatment of viruses, using inactivation, removal, or a department-approved combination of 4-log virus inactivation and removal before or at the first customer, shall meet all the microbiological source water monitoring requirements under s. NR 809.325.

NR 809.32(3)(c) (c) Groundwater systems that have fecally contaminated source waters, as determined by source water monitoring conducted under s. NR 809.325, or have significant deficiencies that are identified by the department or that are identified by EPA, under Subpart S of 40 CFR part 141 of the U.S. Code, shall meet the treatment technique requirements in s. NR 809.327. Groundwater systems with fecally contaminated source water or with significant deficiencies are subject to the treatment technique requirements of s. NR 809.327 and shall implement one or more of the following corrective action options:

NR 809.32(3)(c)1. 1. Correct all significant deficiencies.

NR 809.32(3)(c)2. 2. Provide an alternate source of water.

NR 809.32(3)(c)3. 3. Eliminate the source of contamination.

NR 809.32(3)(c)4. 4. Provide treatment that reliably achieves at least 4-log treatment of viruses, before or at the first customer.

NR 809.32(3)(d) (d) Groundwater systems that provide at least 4-log treatment of viruses before or at the first customer shall conduct compliance monitoring to demonstrate treatment effectiveness, as required under s. NR 809.327 (3).

NR 809.32 History History: CR 09-073: cr. Register November 2010 No. 659, eff. 12-1-10.

NR 809.323 NR 809.323 Analytical requirements for groundwater source microbiological contaminants.

NR 809.323(1) (1) Analytical methods.

NR 809.323(1)(a) (a) A water supplier for a groundwater system subject to the source water monitoring requirements of s. NR 809.325 (2) shall collect a standard sample volume of at least 100 mL for E. coli analysis regardless of the analytical method used.

NR 809.323(1)(b) (b) A water supplier for a groundwater system shall analyze all groundwater source samples, collected under s. NR 809.325 (2), using one of the analytical methods listed in Table G for the presence of E. coli. If the department requires a public water system to be tested for the presence of enterococci or coliphage, the water supplier shall use one of the methods listed in Table G.

NR 809.323(1)(c) (c) Sample volumes of at least 100 mL shall be used for all analyses using the methods in Table G. Analyses shall be conducted in accordance with the documents listed in the footnotes to Table G.

NR 809.323(1)(d) (d) The sample holding time shall not exceed 30 hours. In all cases, samples should be analyzed as soon after collection as possible.

NR 809.323 Note Note: Copies of the documents listed in the footnotes may be obtained from the sources listed in the footnotes. Copies may be inspected at EPA’s Drinking Water Docket, EPA West, 1301 Constitution Avenue, NW., Room B102, Washington DC 20460, Telephone: [phone], or at the National Archives and Records Administration (NARA). For information on the availability of this material at NARA, call 202-741-6030, or go to: http://www.archives.gov/federal_register/code_of_federal_regulations/ibr_locations.html.

1   Methods are described in Standard Methods for the Examination of Water and Wastewater 20th edition (1998) and copies may be obtained from the American Public Health Association, 1015 Fifteenth Street, NW., Washington, DC 20005-2605.

2   Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092.

3   EPA Method 1604: Total Coliforms and Escherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (MI Medium); September 2002, EPA 821-R-02-024. Method is available at http://www.epa.gov/microbes/documents/1604sp02.pdf or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

4   A description of the m-ColiBlue24 Test, “Total Coliforms and E. coli Membrane Filtration Method with m-ColiBlue24Ò Broth,” Method No. 10029 Revision 2, August 17, 1999, is available from Hach Company,100 Dayton Ave., Ames, IA 50010 or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

5   A description of the E*Colite Test, “Charm E*Colite Presence/Absence Test for Detection and Identification of Coliform Bacteria and Escherichia coli in Drinking Water,” January 9, 1998, is available from Charm Sciences, Inc., 659 Andover St., Lawrence, MA 01843-1032 or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

6   EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be used for E. coli testing step as described in Sec. 141.21(f)(6)(i) or (ii) after use of Standard Methods 9221 B, 9221 D, 9222 B, or 9222 C.

7   EPA Method 1600: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-[beta]-D-Glucoside Agar (mEI) EPA 821-R- 02-022 (September 2002) is an approved variation of Standard Method 9230C. The method is available at http://www.epa.gov/microbes/documents/1600sp02.pdf or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

8   Medium is available through IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, Maine 04092. Preparation and use of the medium is set forth in the article “Evaluation of Enterolert for Enumeration of Enterococci in Recreational Waters,” by Budnick, G.E., Howard, R.T., and Mayo, D.R., 1996, Applied and Environmental Microbiology, 62:3881-3884.

9   EPA Method 1601: Male-specific (F+) and Somatic Coliphage in Water by Two-step Enrichment Procedure; April 2001, EPA 821-R-01-030. Method is available at http://www.epa.gov/microbes/documents/1601ap01.pdf or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

10 EPA Method 1602: Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure; April 2001, EPA 821-R-01-029. Method is available at http://www.epa.gov/microbes/documents/1602ap01.pdf or from EPA’s Water Resource Center (RC-4100T), 1200 Pennsylvania Avenue, NW., Washington, DC 20460.

NR 809.323(2) (2) Invalidation of an E. coli-positive groundwater source sample.

NR 809.323(2)(a) (a) The department may invalidate an E. coli-positive groundwater source sample collected under s. NR 809.325 (2) under the conditions specified in subd. 1. or 2.